WG 5: Microvascular Networks

Microvascular Networks

The goal of the Microvascular Networks research is to design and construct functional microvascular networks for use in the next generation of biological machines. 

These networks must be large enough to require a circulation in order to meet the metabolic needs of the other cell types. Currently, differentiated cell lines are being used in microfluidic devices to create networks that span a distance of up to 1 mm and can be perfused from side channels. Mouse ES cells and mouse and human MSCs are also being developed that could be used both for the endothelial network and for the pericytes and smooth muscle cells presumably needed for long-term viability and phenotypic stability.

 Image 1. 10X Phase 01 w/ scaleImage 2.  10X Phase 02 w/ scaleImage 3. 20X Confocal 01Image 4. 60X Confocal 02

Engineered, perfusable microvascular networks on a microfluidic chip.  Endothelial cells cultured in the presence of normal human lung fibroblasts spontaneously form microvascular networks in a three dimensional hydrogel.  

Kamm RD, Bashir R. Creating living cellular machines. Ann Biomed Eng. 2014;42(2):445-59.
Bao G, Nerem RM. Frontiers in Bioengineering Research 42(2): 241-242, 2014. Annals of Biomedical Engineering . 2014;.
Uzel SGM, Pavesi A, Kamm RD. Microfabrication and microfluidics for muscle tissue models. Progress in Biophysics and Molecular Biology. 2014;.
Nerem RM. Stem Cell Engineering. Tissue Engineering, Part A. 2014;20(5-6):893-894.
Wong KHK, Chan JM, Kamm RD, Tien J. Microfluidic models of vascular functions. Annu Rev Biomed Eng. 2012;14:205-30.